Mechanisms of establishment of persistent SARS-CoV-infected cells.
Identifieur interne : 003E23 ( Main/Exploration ); précédent : 003E22; suivant : 003E24Mechanisms of establishment of persistent SARS-CoV-infected cells.
Auteurs : Tetsuya Mizutani [Japon] ; Shuetsu Fukushi ; Koji Ishii ; Yuko Sasaki ; Tsuyoshi Kenri ; Masayuki Saijo ; Yumi Kanaji ; Kinji Shirota ; Ichiro Kurane ; Shigeru MorikawaSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2006.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : Cytokines, Syndrome respiratoire aigu sévère.
- physiologie : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Apoptose, Cellules Vero, Survie cellulaire, Techniques de culture cellulaire.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Cytokines.
- metabolism : Severe Acute Respiratory Syndrome.
- methods : Cell Culture Techniques.
- physiology : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Apoptosis, Cell Survival, Chlorocebus aethiops, Vero Cells.
Abstract
Previously, we reported the establishment of cells with persistent SARS-CoV infection after apoptotic events and showed that both JNK and PI3K/Akt signaling pathways are important for persistence by treatment with inhibitors at the early stages of SARS-CoV infection. However, the mechanisms of establishment of persistent infection are still unclear. In this study, we investigated which signaling pathways play important roles in escape from apoptosis in cells infected with SARS-CoV. In persistently infected cells at 50h.p.i., PI3K/Akt, JNK, p38 MAPK and Bcl-2 were phosphorylated and the protein levels of Bcl-2 and Bcl-xL were increased. When surviving cells were treated with the JNK-specific inhibitor, SP600125, at 50h.p.i., all cells died, suggesting that the JNK signaling pathway is necessary for maintenance of persistently infected cells. Among the signaling pathways in persistently infected cells, Akt and JNK were phosphorylated in SARS-CoV-nucleocapsid (N) protein-expressing Vero E6 cells using vaccinia viral vector (DIs), strongly suggesting that N protein-induced phosphorylation of Akt and JNK are necessary to establish persistence. These results indicated that at least four proteins, Akt, JNK, Bcl-2 and Bcl-xL, are necessary for survival of persistently SARS-CoV-infected cells.
DOI: 10.1016/j.bbrc.2006.06.086
PubMed: 16808902
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002186
- to stream PubMed, to step Curation: 002186
- to stream PubMed, to step Checkpoint: 002114
- to stream Ncbi, to step Merge: 001555
- to stream Ncbi, to step Curation: 001555
- to stream Ncbi, to step Checkpoint: 001555
- to stream Main, to step Merge: 004004
- to stream Main, to step Curation: 003E23
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mechanisms of establishment of persistent SARS-CoV-infected cells.</title><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation wicri:level="3"><nlm:affiliation>Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan. tmizutan@nih.go.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name></author><author><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name></author><author><name sortKey="Sasaki, Yuko" sort="Sasaki, Yuko" uniqKey="Sasaki Y" first="Yuko" last="Sasaki">Yuko Sasaki</name></author><author><name sortKey="Kenri, Tsuyoshi" sort="Kenri, Tsuyoshi" uniqKey="Kenri T" first="Tsuyoshi" last="Kenri">Tsuyoshi Kenri</name></author><author><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name></author><author><name sortKey="Kanaji, Yumi" sort="Kanaji, Yumi" uniqKey="Kanaji Y" first="Yumi" last="Kanaji">Yumi Kanaji</name></author><author><name sortKey="Shirota, Kinji" sort="Shirota, Kinji" uniqKey="Shirota K" first="Kinji" last="Shirota">Kinji Shirota</name></author><author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16808902</idno><idno type="pmid">16808902</idno><idno type="doi">10.1016/j.bbrc.2006.06.086</idno><idno type="wicri:Area/PubMed/Corpus">002186</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002186</idno><idno type="wicri:Area/PubMed/Curation">002186</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002186</idno><idno type="wicri:Area/PubMed/Checkpoint">002114</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002114</idno><idno type="wicri:Area/Ncbi/Merge">001555</idno><idno type="wicri:Area/Ncbi/Curation">001555</idno><idno type="wicri:Area/Ncbi/Checkpoint">001555</idno><idno type="wicri:doubleKey">0006-291X:2006:Mizutani T:mechanisms:of:establishment</idno><idno type="wicri:Area/Main/Merge">004004</idno><idno type="wicri:Area/Main/Curation">003E23</idno><idno type="wicri:Area/Main/Exploration">003E23</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mechanisms of establishment of persistent SARS-CoV-infected cells.</title><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation wicri:level="3"><nlm:affiliation>Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo, Japan. tmizutan@nih.go.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Virology 1, National Institute of Infectious Diseases, Musashimurayama, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name></author><author><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name></author><author><name sortKey="Sasaki, Yuko" sort="Sasaki, Yuko" uniqKey="Sasaki Y" first="Yuko" last="Sasaki">Yuko Sasaki</name></author><author><name sortKey="Kenri, Tsuyoshi" sort="Kenri, Tsuyoshi" uniqKey="Kenri T" first="Tsuyoshi" last="Kenri">Tsuyoshi Kenri</name></author><author><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name></author><author><name sortKey="Kanaji, Yumi" sort="Kanaji, Yumi" uniqKey="Kanaji Y" first="Yumi" last="Kanaji">Yumi Kanaji</name></author><author><name sortKey="Shirota, Kinji" sort="Shirota, Kinji" uniqKey="Shirota K" first="Kinji" last="Shirota">Kinji Shirota</name></author><author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name></author></analytic><series><title level="j">Biochemical and biophysical research communications</title><idno type="ISSN">0006-291X</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Cell Culture Techniques (methods)</term><term>Cell Survival</term><term>Chlorocebus aethiops</term><term>Cytokines (metabolism)</term><term>SARS Virus (physiology)</term><term>Severe Acute Respiratory Syndrome (metabolism)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Cytokines (métabolisme)</term><term>Survie cellulaire</term><term>Syndrome respiratoire aigu sévère (métabolisme)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Techniques de culture cellulaire ()</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Cell Culture Techniques</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytokines</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Cell Survival</term><term>Chlorocebus aethiops</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Survie cellulaire</term><term>Techniques de culture cellulaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Previously, we reported the establishment of cells with persistent SARS-CoV infection after apoptotic events and showed that both JNK and PI3K/Akt signaling pathways are important for persistence by treatment with inhibitors at the early stages of SARS-CoV infection. However, the mechanisms of establishment of persistent infection are still unclear. In this study, we investigated which signaling pathways play important roles in escape from apoptosis in cells infected with SARS-CoV. In persistently infected cells at 50h.p.i., PI3K/Akt, JNK, p38 MAPK and Bcl-2 were phosphorylated and the protein levels of Bcl-2 and Bcl-xL were increased. When surviving cells were treated with the JNK-specific inhibitor, SP600125, at 50h.p.i., all cells died, suggesting that the JNK signaling pathway is necessary for maintenance of persistently infected cells. Among the signaling pathways in persistently infected cells, Akt and JNK were phosphorylated in SARS-CoV-nucleocapsid (N) protein-expressing Vero E6 cells using vaccinia viral vector (DIs), strongly suggesting that N protein-induced phosphorylation of Akt and JNK are necessary to establish persistence. These results indicated that at least four proteins, Akt, JNK, Bcl-2 and Bcl-xL, are necessary for survival of persistently SARS-CoV-infected cells.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name><name sortKey="Ishii, Koji" sort="Ishii, Koji" uniqKey="Ishii K" first="Koji" last="Ishii">Koji Ishii</name><name sortKey="Kanaji, Yumi" sort="Kanaji, Yumi" uniqKey="Kanaji Y" first="Yumi" last="Kanaji">Yumi Kanaji</name><name sortKey="Kenri, Tsuyoshi" sort="Kenri, Tsuyoshi" uniqKey="Kenri T" first="Tsuyoshi" last="Kenri">Tsuyoshi Kenri</name><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name><name sortKey="Sasaki, Yuko" sort="Sasaki, Yuko" uniqKey="Sasaki Y" first="Yuko" last="Sasaki">Yuko Sasaki</name><name sortKey="Shirota, Kinji" sort="Shirota, Kinji" uniqKey="Shirota K" first="Kinji" last="Shirota">Kinji Shirota</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003E23 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003E23 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:16808902
|texte= Mechanisms of establishment of persistent SARS-CoV-infected cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16808902" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |